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More than 600 feed ingredients mold content analysis, the results were shocking!

Reading times:12637   Release time:2017-9-25 9:25:02  author:Chinese Tianyu group

Fungal toxin

Mycotoxins are produced by different filamentous fungal secondary metabolism of toxic substances, the crops grown in the field, feed processing and storage process, the constant changes of the environment will cause fungal growth and metabolism of a large number of fungus toxin. The pollution of the mould toxin not only brings harm to the animals, but also has a potential threat to human health. Analysis of the Biological Technology Co., Ltd., Jiangsu Alvarez & Marsal experiment center of 2014 feed and raw material samples of mycotoxin detection results are statistical analysis, and the formation of the report, to provide a reference for colleagues in the industry.

1. Sample overview: from January to December 2014, Biological Technology Co., Ltd., Jiangsu Alvarez & Marsal received a total of from Jiangsu, Sichuan, Beijing, Shandong, Jiangxi, Henan, Qingdao, Anhui, Zhejiang, Shanghai and other regions of the sample of 612, among the 38 samples of corn 138, corn byproducts 148, wheat and by-products 42, cake and meal, full price feed 168 and other 78 samples. Test analysis center of the 612 samples were carried out in the detection of aflatoxin, corn, and vomiting toxin.

2. Methods: samples were detected by ELISA ELISA kit. Different toxin detection kit to detect the corresponding toxin. In order to get the best results, and experimental analysis center also of different manufacturers kit comparison, screening, and kit for the measured results and liquid phase chromatography results were compared, and selects the most appropriate kit.

Solid phase direct competitive enzyme linked immunosorbent assay was used in ELISA kit. In coated with porous corresponding toxin antibody added standard or by extracting solution treated samples and enzyme standard, standard or sample of toxin and toxin conjugates antibody competition, wash away the unbound enzyme markers, TMB substrate color, adding the stop solution after using a microplate reader at 450 nm wavelength were detected. The maximum limit of the various fungal toxins are: Aflatoxin 20ppb, corn, 500ppb, 1000ppb, vomiting toxin.

3. detection result:

3-1 overall condition of sample: in 2014 received all the samples were detected mycotoxins, figure 1 can be seen from the detected a mycotoxin samples at 0 and detected eight samples of both toxins, accounting for 1.31% of the total number of samples detected three accounted for 98.69%. Therefore, these three kinds of fungal toxins in feed and raw materials in the contamination is very common, and there are many kinds of coexistence of fungal toxins.

Fungal toxin

The overall detection of the mold toxin in the sample is shown in Table 1 and figure 2. The positive detection rate of aflatoxin B1 was 98.69%, and the positive rate of the detection rate of 100%, which was, the positive rate of the wheat and the byproduct was the lowest, which was 85.71%. From the absolute content of all kinds of mould toxin, the highest content of aflatoxin 24.27ppb was B1, which was derived from corn starch residue. But the positive samples of aflatoxin B1 average value is only 7.61ppb median (expressed positive samples in 50 per sample toxin content is lower than the value, 50% of the sample is higher than the numerical) for 7.51ppb below the provisions of the state standard of feed hygiene (20 ppb);


The highest levels of the and 4416.57ppb, respectively, were both 1778.52ppb and (far exceeding the maximum limit), both of which were derived from corn protein feed. The mean values of these two toxin levels were 278.47ppb and 858.23ppb, respectively, and the mean values were 246.91pb and 606.13ppb, respectively.


Fungal toxin

Fungal toxin

3-2 corn sample test results: from the table 2 can be seen in the corn samples in the absolute content of aflatoxin B1 is not high, the average and median values were 4.35ppb and 3.08ppb. But the highest content of vomiting toxin reached 4320.92ppb, exceeding the rate reached 19.12%. The mean values of the content of the content of the and the vomiting toxin were 270.14ppb and 844.39ppb respectively. The median values were 225.88ppb and 395.37ppb, which were in the range of feed hygiene standard. Overall, this year, the corn is very serious pollution of vomiting.

Fungal toxin

3-3 corn by-products of sample testing results: corn by-products samples including 72 copies of corn distillers dried grains with solubles, 12 samples of corn protein powder, corn germ meal 44, shortcreting corn skin 16, corn starch residue 4. As shown in Table 3, such samples pollution is more serious, the mycotoxin content is very high, yellow aspergillus toxin B1, corn gibberellic olefine ketone and vomiting toxin content average respectively 11.28ppb, and 1204.17ppb. 404.46ppb, emetic toxin content of the average value has exceeded the maximum limit, the maximum value of corn gluten feed 4416.57ppb originating from. Its excessive rate reached 52.94%. The median values of these three toxins were 10.61ppb, 323.16ppb and 996.81ppb.

Fungal toxin

3-4 wheat and by-products of sample testing results: 4 can be seen from the table, aflatoxin B1 positive detection rate and content were lower. The positive rate was 85.71%, and the average value within a con tent range; zearalenone content was significantly lower than that of maize, the average value for the 154.78ppb; vomiting toxin content is relatively high, the average and median values respectively for 920.75ppb 761.09ppb, mainly is bran pollution more serious.

Fungal toxin

3-5 cake sample test results: can be seen from table 5, the cake samples of aflatoxin B1 content is higher than corn, the highest value of 18.95ppb, from the peanut meal. The mean values of the three toxin levels were 11.58ppb, 197.88ppb and 392.27ppb, respectively. The mean values were 12.40ppb, 211.30ppb and 381.05ppb, respectively. Overall, the cake meal samples mainly aflatoxin contamination is slightly worse.

Fungal toxin

3-6 full price material sample test results: can be seen from table 6, the positive samples of aflatoxin B1 average and median values were 7.91 and 7.67, lower than the national standard (20ppb). The positive detection rates of the corn and the vomiting toxin were 100%, the average values were 278.37ppb and 885.48ppb. Vomiting toxin content is very high, the highest value reached 3653.51ppb, exceeding the rate of 11.9%. Median values were 251.16ppb and 564.17ppb. This shows that in 2014 the full price of the material sample mold toxin pollution situation is serious.

Fungal toxin

3-7 sample testing results: from absolute content of view (Figure 3 and Figure 4), corn by-products samples of three kinds of toxin content of average was the highest; wheat and its by-products samples of aflatoxin B1 and corn gibberellic olefine ketone content mean values were relatively low, but the emetic toxin content is high; cake and meal samples of Aspergillus flavus toxin B1 content higher, corn and the full price of feed corn gibberellic olefine ketone and emetic toxin content is slightly higher, toxin B1 pollution was more serious than Aspergillus flavus.

Fungal toxin

4. Summary and conclusion: from the above test results can be seen that the occurrence probability of aflatoxin B1 is lower than that of the corn and the vomiting toxin, and the pollution degree is not high. Among them, the cake meal of aflatoxin contamination slightly heavier.

The positive detection rate of the corn, red and green and vomiting toxin was higher, but the pollution degree of the corn was lower than that of the vomiting toxin, and the pollution of the vomiting toxin was more serious. Corn, the full price of feed of the emetic toxin content is very high, and the pollution degree of wheat bran samples decreased, but still to emetic toxin pollution is the most serious. Corn by-products of mycotoxin contamination has been very serious, especially a high content of corn gibberellic olefine ketone and emetic toxin in the materials and samples of above of half the number of emetic toxin content beyond the standard of feed hygiene limited and need to strengthen efforts to detect.

Overall, the 2014 feed and raw material aflatoxin contamination is not the most important issue, and the serious pollution of the corn, red and green, especially the corn and the full price. Therefore, it is necessary to detect the feed and raw material for the detection of fungal toxin regularly, and can take the corresponding measures such as adding the mold toxin adsorbent in the feed, so as to reduce the loss caused by the fungus toxin. The data reported by aomai biotechnology experimental analysis center issued

Chinese Tianyu group copyright.Reproduced please indicate the source.

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